You may find that no matter how many times you enable your Mixer connection, it immediately disables itself automatically. This almost always means Botisimo has been banned from your channel. Also, note that if you are costreaming then Botisimo may have been banned by the costreamer as well. If Botisimo is banned in the costreamer's channel then while you are costreaming with them it is as though Botisimo is also banned in your channel.
Here's what you can do about it
1. Unban Botisimo from chat(s) and make it a moderator
2. Reenable the Mixer connection on your Connections Page
Customer support service by UserEcho